labelling technique造句
例句與造句
- By using the gfp - labeling technique , we have found that gfp - cam colocalized with all the characterized structures formed during cytokinesis
通過采用gfp標(biāo)記技術(shù)，我們觀察到gfp - cam與胞質(zhì)分裂期細(xì)胞內(nèi)形成的特征結(jié)構(gòu)存在共分布。 - But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area " . to answer these questions , we observed systemically the synaptic connections among the pv - like immunoreactive neurons , fibers and terminals and the connections between dnpi - like , gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry , triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique
但是目前對(duì)新發(fā)現(xiàn)的囊泡膜mu轉(zhuǎn)運(yùn)體一dnn樣陽性終末與帶狀區(qū)內(nèi)pag樣陽性神經(jīng)元之間ej關(guān)系，以及谷氨酸脫發(fā)酶（ gad ，是gaba能神經(jīng)元和終末的特異性標(biāo)識(shí)物）是否參與其調(diào)控作用，尚缺乏系統(tǒng)的形態(tài)學(xué)資料。 - The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation . 2 . following lps or seb was administered intraperitoneally , the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice . double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔內(nèi)給予細(xì)菌內(nèi)毒素lps或腸毒素seb ，用免疫組織化學(xué)方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達(dá)，并采用雙標(biāo)記技術(shù)觀察了1型il刁受體陽性神經(jīng)元和加壓素及催產(chǎn)素表達(dá)的關(guān)系。 - The amplification system was optimized so that the pcr with different primers can be carried out under the same condition . the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques . sequences were analyzed and compared base on sequencing analysis3 . 4 and seq / ede software
方法根據(jù)mtdna控制區(qū)及其周圍區(qū)域的序列，設(shè)計(jì)多對(duì)引物，探索優(yōu)化擴(kuò)增體系，使擴(kuò)增條件能夠同時(shí)滿足多對(duì)引物的需要，用sanger末端終止法及熒光標(biāo)記技術(shù)對(duì)樣本進(jìn)行dna測序， sequencinganalysis3 . 4和seq ede軟件進(jìn)行序列分析和比對(duì)。 - The characteristics of this method are : a , directly counting cell number without the influence of the metabolic state of the cells ; b , discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c , less treatment step , and free - radioactivity ; d , high sensitivity and reliability . 2 , using the above assay , immunofluorescent labeled technique , and flow cytometry , the pbmc proliferation , apoptosis , necrosis , cell cycle , activation , cytokines and membrane marker were detected . the results showed that the number of pbmc reduced , but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle , but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結(jié)合以上創(chuàng)建的方法和免疫熒光流式細(xì)胞術(shù)，用k562細(xì)胞株可溶性分泌物(上清)對(duì)外周血單個(gè)核細(xì)胞( pbmc )進(jìn)行培養(yǎng)以模擬體內(nèi)微環(huán)境，然后分別從細(xì)胞增殖、凋亡、壞死、細(xì)胞周期、活性、細(xì)胞因子和表面抗原表達(dá)等方面進(jìn)行研究，結(jié)果發(fā)現(xiàn)用腫瘤上清培養(yǎng)的pbmc細(xì)胞數(shù)量下降明顯，但同時(shí)對(duì)其有激活作用，且呈劑量依賴性；細(xì)胞數(shù)的下降主要是由細(xì)胞壞死和凋亡引起的，腫瘤上清對(duì)細(xì)胞周期沒有阻斷作用，反而略有促進(jìn)作用； t細(xì)胞亞群比例增加，并促進(jìn)表達(dá)th1 、 th2細(xì)胞因子。 - It's difficult to find labelling technique in a sentence. 用labelling technique造句挺難的
- The self - segregation behavior of amphiphilic copolymer on pdl - la scaffold was investigated via fluorescence - labeling technique . the modified scaffold with hydrophilic surface will not only favor the penetration of cell suspension and culture medium , but also provide the microenvironment for the growth of cells with the peo spacer combining amino acid ( rgd ) structure . according the above result , the cytocompatibility test was also performed on pdl - la 3d scaffold modified by amphiphilic copolymer with alkaline amino acid end
這種親水表面不僅有利于細(xì)胞懸液和培養(yǎng)介質(zhì)的進(jìn)入，并可以通過peo橋聯(lián)的氨基酸( rgd )為細(xì)胞在三維多孔支架內(nèi)的生長提供類細(xì)胞外基質(zhì)環(huán)境；根據(jù)以上結(jié)果，本文對(duì)堿性氨基酸為peo鏈端基的兩親共聚物-氨基酸類細(xì)胞外基質(zhì)修飾的聚乳酸三維支架進(jìn)行了細(xì)胞相容性的測試。 - However , the advance of intracellular labeling techniques enables us not only to visualize more complete dendritic arbor for qualitative analysis , but also to examine the relation between changes in the dendritic arborization and the evoked fast postsynaptic curents - 3 - ( fpscs ) in the same neurons during the postnatal development the aim of this study was to systematically examine the postnatal changes in the configuration of fpscs evoked by the focal stimulation of the stratum radiatum of the ca1 region , and the relationship between the dendritic arborization and evoked fpscs in the rat hippocampal ca1 pyramidal neurons using whole - cell blind patch recording technique combined with biocytin intracellular labeling during the postnatal development ( postnatal day 2 - 70 , p2 - p70 )
但是，細(xì)胞內(nèi)染色技術(shù)的進(jìn)步使我們不僅能觀察到更完整的樹突分支來用于定性研究，而且也可以在同一神經(jīng)元上研究在發(fā)育過程中樹突分支的變化與誘發(fā)的快突觸后電流( fastpostsynapticcurrents , fpscs )之間的關(guān)系。因此，本研究應(yīng)用盲法腦片膜片鉗記錄并結(jié)合biocytin細(xì)胞內(nèi)染色方法，對(duì)發(fā)育過程中(生后2 70天)局部刺激大鼠海馬ca1區(qū)輻射層在錐體神經(jīng)元誘發(fā)的fpscs的成分變化，以及ca1錐體神經(jīng)元的樹突分支與誘發(fā)的fpscs的關(guān)系進(jìn)行了較為系統(tǒng)的研究。